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manP [2019-09-06 10:35:21]
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manP [2019-09-06 10:35:21]

mannose-specific permease of the phosphotransferase system, EIIBCA of the PTS, Trigger enzyme, control of ManR activity
Locus
BSU_12010
Isoelectric point
6.72
Molecular weight
61.81 kDa
Protein length
650 aa Sequence Blast
Gene length
1953 bp Sequence Blast
Function
mannose uptake and phosphorylation, control of ManR activity
Product
mannose-specific PTS, EIIBCA
Essential
no
Synonyms
yjdD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Phosphotransferase system → Sugar specific PTS proteins
  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of mannose
  • Information processing → Regulation of gene expression → Transcription factors and their control → Control of PRD-type regulators
  • Information processing → Regulation of gene expression → Trigger enzyme → Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Cys residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a His residue
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    1,272,725 1,274,677

    The protein

    Catalyzed reaction/ biological activity

  • transport and concomitant phosphorylation of mannose
  • D-mannose + Nπ-phospho-L-histidyl-[protein] --> D-mannose 6-phosphate + L-histidyl-[protein] (according to UniProt)

    • Protein family

  • PTS permease, fructose/ mannitol family PubMed

    • Paralogous protein(s)

  • FruA

    • Domains

  • PTS EIIC domain type-2 (aa 123-456) (according to UniProt)

    • Modification

  • phosphorylation on Ser-365 PubMed

    • Structure

  • 2R48 (IIA domain)
  • 2R4Q (FruA, IIB domain, 56% identity)

    • Localization

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    manP-manA-yjdF
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • ManR: activation, in the presence of mannose and absence of glucose PubMed, in ManR regulon
  • RNA switch: termination/antitermination, expession may be controlled by a potential RNA switch located in the 5' untranslated region of the manP-manA-yjdF mRNA between manA and yjdF, in RNA switch

    • Regulation

  • induced by mannose (ManR) PubMed
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    Biological materials

    Mutant

  • MGNA-A270 (yjdD::erm), available at the NBRP B. subtilis, Japan
  • BKE12010 (manP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA, downstream forward: _UP4_ATCGAATAAAGCGGGGGATT
  • BKK12010 (manP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGGAAACCTCCTTTAAA, downstream forward: _UP4_ATCGAATAAAGCGGGGGATT

    • References

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